gram stain

Peter Herman rpeter at
Thu Apr 16 09:30:50 EST 1998

tan poh ewe wrote:
> Can anyone tell me the mechanism of heat fixing in a smear like gram stain.
> How does heating help fix the material on the slide????
> Thank you.
> tpe

The analogy I always use for class is an egg in a frying pan with no
butter.  If you heat it enough it sticks.  If you heat it to much it
chars. If you heat it before it is dry you poach it and the cell (egg)
is denatured without sticking.


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