HPLC columns attachment

Thierry Mann tmann at theriver.com
Thu Apr 23 10:13:49 EST 1998

Emir KHATIPOV wrote:

> Could anyone clear up the following.
> Does one have to follow the same precautions when attaching/detaching HPLC
> columns, as in the case of low pressure column chromatography, to avoid air
> bubble penetration into the column? I mean do I have to first disconnect
> upper side then lower, paying attention to always feed junctions with some
> extra buffer before screwing on a nut, and connect the column reversely
> first by lower, then by upper end? I could not find such recommendations or
> precautions anywhere. Looks like HPLC might have a certain protection
> (bubble traps?...).
> Please reply to em at unforgettable.com if possible. Thank you.
> Emir

I really wouldn't advise storing your columns in buffer. Store them in
acetonitrile ormethanol. As far as I know, there is no magic trick to
installing an HPLC column. You should
however work with de-gassed solvents while running.

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