HPLC columns attachment

Peter pxpst2 at unixs.cis.pitt.edu
Thu Apr 23 18:04:39 EST 1998

In article <6hmgjg$93g$1 at rpnntp.aist.go.jp>, "Emir KHATIPOV"
<khatipov at nibh.go.jp> wrote:

> Could anyone clear up the following.
> Does one have to follow the same precautions when attaching/detaching HPLC
> columns, as in the case of low pressure column chromatography, to avoid air
> bubble penetration into the column? I mean do I have to first disconnect
> upper side then lower, paying attention to always feed junctions with some
> extra buffer before screwing on a nut, and connect the column reversely
> first by lower, then by upper end? I could not find such recommendations or
> precautions anywhere. Looks like HPLC might have a certain protection
> (bubble traps?...).
> Please reply to em at unforgettable.com if possible. Thank you.
> Emir

Don't want to sound like an ass but I will...

"they don't call then zero volume junctions for nothing"


"Don't you eat that yellow snow
            watch out where the Huskies go"    FZ


More information about the Microbio mailing list