Winogradsky Columns

Stephen W. Mamber smamber at usccmail.bms.com
Thu Aug 6 14:02:51 EST 1998


Why not simply add some agar, like maybe 0.5 - 0.7%?  SWM

Martin Weiss wrote:
> 
>     Has anyone experience setting up Winogradsky colums using only
> water? Can you get a stratified column of anerobic bacteria grwoing in a
> column of  water in the same way that you can with a column of mud? I am
> trying by building a small classical column, about 25% of the total
> volume of the column, (using mud mixed with egg or wood chips or paper)
> and the rest of the column water collected with the mud. So far, about a
> week no luck.
> 
> I am hoping the colonies would be more visible and perhaps we could
> sample more easily, using a thin plastic tube, used to cover fluorescent
> light bulbs.
> 
> Any ideas or suggestions?
> 
> Cheers,
> 
> Martin Weiss, Ph.D.
> Bilogy Director
> New York Hall of Science
> http://www.nyhallsci.org



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