shiga toxin assay

wayne.conlan at nrc.ca wayne.conlan at nrc.ca
Thu Dec 10 16:53:35 EST 1998


I'm looking for a cheap and reliable assay to determine whether some E. coli
O157:H7 strains I have are producing SLT I and, or SLT II.  At this stage I'm
not looking for a functional assay, just something to say whether these
toxins are present or not in brain heart broth culture supernatant in which
the organism actively grew for a period of 4-5 hrs.  Any advice would be
gratefully received.

Thanks,

Wayne


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