Is it possible to clear plasmids from A.tumifaciens?

Kevin L. Reed kevinlreed at email.msn.com
Fri Feb 6 02:28:28 EST 1998


I have a wild type strain of Agrobacterium tumifaciens and was wondering if
it is possible to remove all plasmid DNA while leaving bacterial
housekeeping DNA intact or if any one has protocols for homologous gene
insertion in 200,00bp wild type A.tumifaciens genome. Any thoughts are
appreciated.
Kevin Reed
KevinLReed at msn.com





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