Mycoplasma media

Robert Randolph rrandolph at
Thu Jan 1 21:28:33 EST 1998

Randolph Biomedical makes a complete line of prepared mycoplasma
media, supplements, enrichemnts and selective agents for the media.
Their webpage is:
401-826-1407 * microbiology at

Robert Randolph

On 27 Dec 1997 08:02:19 -0800, yersinia at CYBERNEX.NET (Yersinia) wrote:

>Mark Wolcott wrote,
><Does anyone know of a commercial source for Mycoplasma media? I'm 
>particularly interested in SP4 or Jordan's formulations.>
>Difco sells PPLO media for the growth of  "pleuro-pneumonia-like 
>organisms," i.e., mycoplasma. However, it doesn't say anything about SP4 
>or Jordan's formulations (and alas, I'm not familiar with those either, 
>not having worked with mycoplasma myself). I can't judge, therefore, if 
>this is the media you want, but perhaps you can from the recipes:
>Per Liter of DI H20:
>PPLO Agar:
>Beef Heart Infusion:  50 g
>Peptone: 10 g
>NaCl: 5 g
>Agar: 14 g
>PPLO Broth Media:
>Formulations same as above, minus the agar. Available with and without 
>crystal violet.
>PPLO Serum fraction (sterile solution of partially purified serum 
>fraction required for growth of mycoplasma)
>Well, I hope this helps you a little.
>"Van der waals with boughs of holly..."

rrandolph at

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