Probes for nitrate-reducers
ant01 at globalnet.co.uk
Thu Jan 15 08:31:22 EST 1998
I am a PhD student studying hydrogen-utilising bacteria in the human
colon. I have isolated disimilatory nitrate-reducing species from faeces
using batch and chemostat culture. I have found a predominance of
E.coli, but I am not sure if this is just down to my culture conditions
(I have used gentamycin and naladixic acid to inhibit E.coli). I need a
cross-reference technique to determine whether E.coli is the predominant
nitrate reducer. For this Fluorescent in situ hybridisation (FISH) would
be ideal, but I am not sure how to probe for total nitrate reducers.
Does anyone have any ideas? I thought about Nitrate reductase or Nitrite
reductase, but I don't know how conserved these genes are in NRB's.
Please e-mail me at: nick.parham at BBSRC.ac.uk
Thanks in advance, Nick.
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