Low-protease strain of B. subtilis

Arul Jayaraman arul at design.eng.uci.edu
Thu Jan 15 17:00:39 EST 1998


Hi
I have cloned a small peptide into an E. coli/Bacillus shuttle vector.
I use a ATCC strain of B. subtilis (168) and B. subtilis BE1500 and it
appears that the peptide is getting degraded due to protease activity.
Can anyone point me to a reference for a B. subtilis strain with low
protease activity, which I can transfer my plasmid into?
 tried bacillus brevis as it is supposed to have low protease activity;
but, transforming it is very difficult.
Thanks a lot
Arul

---------------------------------------------------------------
Arul Jayaraman
Dept. of Chemical & Biochemical Engineering
University of California, Irvine
Irvine, CA 92697-2575
Ph : (714)-509-1060 (H)
     (714)-824-8389 (L)
     (714)-824-2541 (Fax)
http://www.eng.uci.edu/~arul/

       





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