Preservation of transformed E. coli

Yersinia yersinia at CYBERNEX.NET
Mon Jun 1 19:22:43 EST 1998

Hello all,

A  correspondent of mine who's a graduate student asked me a question, 
but some of it's slightly over my head, so I thought I'd pass it along to 
you knowledgeable people:

<What's the best temperature at which to keep agar stab cultures?  (I'm 
growing pUC19-transformed E. coli in 2XYT agar with a trace of 
ampicillin).  My lab protocol says to keep 'em at room temperature, but 
I'm worried about the ampicillin stability.  My lab advisor says to stick 
'em in the -20 freezer, but they don't have any cryoprotectants like 
glycerol in them.  At present, I've got them in the fridge at 4 degrees.>

Thank you for your help.


"The lab called. Your brain is ready."

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