Preservation of transformed E. coli
yersinia at CYBERNEX.NET
Mon Jun 1 19:22:43 EST 1998
A correspondent of mine who's a graduate student asked me a question,
but some of it's slightly over my head, so I thought I'd pass it along to
you knowledgeable people:
<What's the best temperature at which to keep agar stab cultures? (I'm
growing pUC19-transformed E. coli in 2XYT agar with a trace of
ampicillin). My lab protocol says to keep 'em at room temperature, but
I'm worried about the ampicillin stability. My lab advisor says to stick
'em in the -20 freezer, but they don't have any cryoprotectants like
glycerol in them. At present, I've got them in the fridge at 4 degrees.>
Thank you for your help.
"The lab called. Your brain is ready."
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