competent cells

Gerd gerdn at ibg.uit.no
Thu Jun 4 02:21:15 EST 1998


John Augliera wrote:
> 
> Does anybody have a method for making bacterial cells competent and t


You need to specify hich type of compent cells you want. Some protocols
uses DMSO in stead of glycerol fro heat-shock cells.
Try this reference:
Inoue, H.,et al. 1990. High efficiency transformation of Escherichia
coli with plasmids. Gene 96: 23-28.

Gerd



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