french press

Peter C. Giardina, Ph.D. pgiardin at blue.weeg.uiowa.edu
Thu Jun 11 15:19:26 EST 1998


On Wed, 10 Jun 1998, Rico Laage wrote:

> Dear colleagues
> 
> I´m starting to use a french press for breaking my bugs to purify a
> recombinant protein, I have a problem what pressure I should use for E.
> coli to completely crack the cells- I am operating aSLM Aminco with the
> 40K manual fill cell- thanx a lot for any suggestions-
> 
> --
> *****************************************************************************************
> 
> Rico Laage
> Neurobiology
> University of Heidelberg
> Im Neuenheimer Feld 364
> 69120 Heidelberg
> Germany
> Voice  +49 (6221)54-4863      Fax    +49 (6221)54-4496
> World-Wide-Web:
> http://server.nbio.uni-heidelberg.de/Neurobiology.html
> http://server.nbio.uni-heidelberg.de/Groups/WWW_Langosch/Rico_Laage.html
> 
> ******************************************************************************************

I use 10,000 psi x2 passes for 10ml of E. coli suspension
..

Hope this helps.


*********************************************************
Peter C. Giardina, Ph.D.
University of Iowa
Dept. of Microbiology
51 Newton Rd.
Iowa City, IA 52242
voice:(319) 353-5654  fax:(319) 335-9006
http://www.geocities.com/CapeCanaveral/Lab/1690/
*********************************************************




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