How do I identify biofilm bacteria?

Robert Randolph rrrandolph at
Wed Jun 24 07:01:47 EST 1998

Start with a good reference. I suggest   Manual of Biocorrosion   by
H.A. Videla, Lewis Publishers.  I find this to be well written and
suitable for the microbiologist and the field engineer. Explains
sample collection, culture methods and media and relationship between
biofilms and microorganisms present.

Randolph Biomedical
microbiology at


On Sun, 21 Jun 1998 17:47:25 GMT, at (JennyG) wrote:

>I need to identify what organisms are in the biofilm of our municipal
>water lines.  Is there any particular sampling techniques I should
>use?  I was planning on just scraping the pipes' walls with a sterile
>spatula and dropping a chunk of the ooze into a sterile container.
>Will this be enough to make sure I can identify it, or should I place
>it onto a nutrient agar on site, without waiting to get back to the
>What do I do once I get to the lab?  I don't know where to start.  All
>I can think of, off the top of my head, is to gram stain the stuff and
>examine morphology.  And maintain a culture of it on nutrient agar.
>I would greatly appreciate any comments or suggestions.
>doublehelix at

w_wallis at

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