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Autoclave Validation

il128 at my-dejanews.com il128 at my-dejanews.com
Thu Jun 25 08:28:52 EST 1998

I have an autoclave validation problem and was hoping to get some input from
my fellow netwise microbiologists.  The cycle is a prevac with a 60 minute
dwell time at 121.1C at 15psi.	The load is made up of 50 bags (Tyvek
Breather bag) of stoppers each bag is about half full. Some of the stoppers
(the indicator stoppers) are sticking together and when they do B.
stearothermophilus spores are surviving.  The stoppers are washed and
siliconized before spiking, bagging and autoclaving.  Any suggestions on how
to prevent the stoppers from sticking together? Paul

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