cRACE mapping of 5' ends bacterial mRNA

nooned at nooned at
Tue Mar 31 06:57:15 EST 1998

  has anyone used the cRACE method of determining 5' start sites of
mRNAs    (Maruyama IN, et al. Nucleic Acids Res. 1995 Sep 25; 23(18):
3796-3797) to map the transcription start sites of mRNAs from bacteria ,
in particular Gram positive bacteria.
And if so, are there any important considerations tips etc.
1. do you need to use 2 sets of nested primers for the 2 inverse PCR steps 
2. what is the best way to remove the template RNA and initial cDNA primer

thanks in advance

david noone
david noone
dept. of genetics
trinity college dublin 
e-mail:nooned at
fax: 01 6798558

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