Thermophilic Bacteria

A.Ferszt a.ferszt at not.ic.ac.uk
Tue Nov 3 16:24:22 EST 1998


Gilbert Douglas wrote:
> 
> I have learned in my Sophomore biology class that one characteristic
> significant to Thermophilic bacteria is the presence of a "Taq" enzyme.  My
> question is, what reaction does this enzyme catalyze in these bacteria and
> why is this enzyme uniquely needed in the PCR?
> 
> I would appreciate any help
> 
> thanks,
> gilbert douglas

the Taq enzyme is a DNA polymerase. It is used in PCR because the
polymerisation can proceed at higher temperatures than ordinary
polymerases, and ceases to work at lower temperatures. The reactions are
done through several heating-cooling cycles to increase yield and
decreases errors.



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