Thermophilic Bacteria

William J. Mason wmason at COMP.UARK.EDU
Wed Nov 4 16:24:56 EST 1998


I believe this is correct.  This is one of the main reasons why they
switched to the Taq polymerase because it is thermostable.  The E.coli
polymerase was not as efficient and tended to denature.

Jeff Mason
University of Arkansas, Biological Sciences/Microbiology
wmason at comp.uark.edu
http://comp.uark.edu/~wmason

On Wed, 4 Nov 1998, David Faguy wrote:

> I thought, when first invented, Mullis didn't use Taq but added Klenow
> fragment of E. coli DNA polymerase every cycle of PCR. Anyone know for
> sure?
> David
> 
> Nicholas Landau wrote:
> > have different versions of DNA polymerase.  Taq polymerase is
> > of consequence to humanity because it was the polymerase used
> > by Mullis et al in the invention of the polymerase chain
> > reaction (PCR), for which Mullis was award the Nobel Prize for
> > Chemistry.
> > 
> 
> -- 
> ********************************************************
> D.M. Faguy, Ph.D.                     e-mail: phage at mscs.dal.ca
> Dept. of Biochemistry                 phone: (902) 494-2968
> Dalhousie University                    fax:     (902) 494-1355
> Halifax, N.S.
> Canada
> ********************************************************
> 
> 




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