How use ultraviolet light to sterilize?

Phil jorge2 at earthlink.net
Sat Nov 7 07:05:07 EST 1998


Jay Mone' wrote:
> 
> Michele,
> The most important factor to consider is the wavelength of light
> emitted by the Hg lamp.  The most germicidal wavelength is 260 nm.
> With that said, I would expect that several hours would be sufficient.
> However, I would check sterility by placing an open petri dish of
> bacterial agar in the box following sterilization, and see if anything
> grows.
> By the way, UV light is the method of choice for sterilizing air.
> 
> Jay Mone'

I'd not depend on UV to "sterlize" anything.  It will lower counts
considerably if used correctly but some bugs are very resistant.
Also be aware that bugs can photoreactivate in white light - i.e.
unrecoverable (in culture) cells exposed to white light can recover
viability.




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