How use ultraviolet light to sterilize?

Dr. Michael J. Miller mignatz at servtech.com
Sat Nov 7 10:38:36 EST 1998


Using UV will reduce the numbers of viable microorganisms in the work
area; however, you will not be sterilizing the air.  Sterilization is a
process that will demonstrate a probability of a non-sterile unit (i.e., 1
in a million), and this is based on validated procedures using biological
indicators and other methods.  Although you will not demonstrate
"sterility", you can show a reduction in airborne counts by placing
fallout plates in the treated area as described in a previously posted
message.  You should leave the light on (in the test chamber) when the
unit is not in use.  To further reduce the risk of contamination,
decontaminate all work surfaces in the test chamber before and after use
with filter-sterilized 70% IPA.  This is a standard practice in micro labs
in the medical device/pharmaceutical indistries.  Hope this helps.

Dr. Michael J. Miller
Director, Biological and Sterilization Sciences
Bausch & Lomb



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