William J. Mason
wmason at COMP.UARK.EDU
Wed Nov 25 10:14:16 EST 1998
Try streaking the bacteria on Baird Parker Medium. This selects for Gram
+ Staph., and the results should allow you to determine if it is coagulase
positive or negative. If it is positive, I would isolate a colony and
streak this on Mannitol Salt Agar. If you get a nice yellow color change,
you have Staph. aureus. If it does not give a color change but does grow,
it could be Staph. epidermidis. My skin micro book says that there are a
diverse number of Coag positive and negative Staphylococcus on the skin.
In addition to this, you can probably isolate Propio. spps. and maybe
some Corynebacterium spp. But I would be very surprised if you have
Pseudomonas (assuming you are studying the resident flora of a WASHED
hand). If not, then your bacteria could be anything that your appendage
with 5 swabs (hand) sampled that day.
Hope this helps,
University of Arkansas, Biological Sciences/Microbiology
wmason at comp.uark.edu
On Wed, 25 Nov 1998, Phil wrote:
> John R. McQuiston wrote:
> > The pink one could be pseudomonas and the white one Staph. aureus or
> > epidermidis. It's hard to tell without more info.
> > In article <Mj%52.6662$DF6.198844 at tor-nn1.netcom.ca>, hluong68 at netcom.ca
> > says...
> > >
> > >I'm conducting an experiment on the type of bacteria on our skin. So far I
> > >have two type of bacteria identified and two's left. The colonies of one of
> > >the types are white, smooth and in a plateau margin. The other type is pink
> > >and has a drop-like margin.
> > >Any help that you can give is appreciated!
> > >Thanks, Horace
> > >
> > >
> I'm sure not familiar with too many pink pseudomonads. To which one(s)
> are you refering?
> Horace, what media are you using?
> Consider that
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