2D Electrophoresis sample prep

Andrew Pridmore andrew at mailbox76.freeserve.co.uk
Thu Nov 26 17:25:03 EST 1998


I have achieved excellent two-dimensional separations of bacterial proteins
using a sample prep described in a Japanese paper, which includes SDS as
well as nonionic detergent in the first dimension sample buffer.

Why does this work?

Surely the negative charge imparted to the proteins by the SDS would disrupt
the isoelectric focussing? Or does the nonionic detergent displace the
negative charge?

Does anyone have a reference which demonstrates what is happening?

Thanks for reading this!

Andrew Pridmore





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