Trimethoprim solution

Alejandro Castillo castillo at tamu.edu
Thu Oct 1 18:24:21 EST 1998


However, lactic acid may cause undesirable bacterial inhibition on the
culture medium. You may consider using 0.1 N hydrochloric acid instead. For
culture media, you dissolve the trimethoprim in a samll amount of HCl and
then adjust the volume with sterile water of phosphate buffer.


Vincent Mulholland wrote in message ...
>According to an old lab manual, trimethoprim should be dissolved in
>1% (w/v) lactic acid at 5 mg/ml for the stock solution and diluted
>100-fold to give the final concentration (e.g. 1 ml in 100 ml broth).
>
>Good luck,
>
>Vince.
>
>
>***************************************************************************
*
>Vincent Mulholland                  Diagnostics & Molecular Biology
Section,
>                                      Scottish Agricultural Science Agency,
>E-Mail: mulholl at sasa.gov.uk             East Craigs,
>Tel:+44(131)2448845 Fax:+44(131)2448912   Edinburgh EH12 8NJ, U.K.
>***************************************************************************
*
>
>
>





More information about the Microbio mailing list