DNA/RNA spectro 260nm

Glen Ulett glen.ulett at jcu.edu.au
Tue Oct 13 00:12:38 EST 1998


When using spectrophotometry at 230/260/280 nm for checking the quantity
and quality of RNA/nucleic acid in a prep, how do interpret readings
which have a very high 230nm value. The ratio of 260/280 is good, ie.
about 2.0 meaning fairly pure nucleic acid, but what if the 230nm
reading is greater than the 260nm reading.? As far as I know, the 230nm
is reading for residual phenol/chloroform/guanidium thiocyanate. If you
can help, please email me, glen.ulett at jcu.edu.au




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