cell staining

Mike O'Hara mjohara at ihug.co.nz
Tue Oct 13 02:14:17 EST 1998


Debra L. Swanson wrote:
> 
> Does anyone have a reliable protocol for cell staining that will differentiate
> viable from nonviable cells?
> Thanks.
> 
> Debra Swanson, Assistant Scientist
> Comparative Medicine
> University of Minnesota
> Minneapolis, MN  55455
> swans061 at maroon.tc.umn.edu


Dear  Debra

The standard technique is to use trypan blue, available from Sigma. For
about 5-10 minutes after mixing (time depends on cell type) the viable
cells remain white or vaguely pink. dead cells turn blue. after a period
or time the dye does get into the live cells.

Mike



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