In situ bacterial DNA quantitation?

Dr. S. Shapiro toukie at zui.unizh.ch
Thu Oct 22 10:26:46 EST 1998


Dear Colleagues;

     I am curious if anyone is aware of a procedure for quantifying the
DNA (or total nucleic acid) in individual bacterial cells that does not
make use of radiosiotopes or flow cytometers.  For example, can it be done
microscopically (electron microscopically) using particular fluorescent
dyes?  Any other suggestions?
 
     Kindly contact me directly at toukie at zui.unizh.ch.  Please provide
reference citations.

Thanks in advance to all responders,
 
S. Shapiro
toukie at zui.unizh.ch

q



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