S.Greve at tu-bs.de
Thu Sep 3 14:12:55 EST 1998
Chris Chilcott wrote:
> Could someone please tell me how long I need to autocalve 5 litres of media
> for, or should I autocalve it in smaller volumes.
> Chris Chilcott
This is from a laboratory manual:
Sterilizationtime 15 minutes at 121°C, 1 bar PLUS heatingtime!
Volume to be sterilized in ml Heatingtime in min
up to 50 5
50 - 100 8
100 - 500 12
500 - 1000 20
Out o fthis you can easily plot a regression curve and extrapolate to
the desired volume.
I hope this helps you.
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