Boris.Braun at post.rwth-aachen.de
Thu Sep 3 05:25:54 EST 1998
Chris Chilcott wrote:
> Could someone please tell me how long I need to autocalve 5 litres of media
> for, or should I autocalve it in smaller volumes.
> Chris Chilcott
i prefer to autoclave my media in 1 litre erlenmeyers. But take care, more than
600mL per bottle will cause overcoocking. If you have a well working autoclav
that gets 2 atm of pressure, 20 minutes at 120°C are long enough to sterilize
all that you want.
More information about the Microbio