autoclave times

Boris Braun Boris.Braun at post.rwth-aachen.de
Thu Sep 3 05:25:54 EST 1998



Chris Chilcott wrote:

> Could someone please tell me how long I need to autocalve 5 litres of media
> for, or should I autocalve it in smaller volumes.
>
> Regards
> Chris Chilcott

  Hi chris,
i prefer to autoclave my media in 1 litre erlenmeyers. But take care, more than
600mL per bottle will cause overcoocking. If you have a well working autoclav
that gets 2 atm of pressure, 20 minutes at 120°C are long enough to sterilize
all that you want.

Cheers,    Boris.




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