single crossover frequency

Gerd gerdn at ibg.uit.no
Mon Sep 7 03:11:10 EST 1998


Hei,
I am trying to do insertion mutagenesis by triparental mating, and is
wondering which frequency of single crossovers might be expected.
Recipient strain: Rhizobium meliloti, 
Donorstrain: DH5alpha with 8 kb plasmid (370 bp "almost homologous"
sequence, reportergene, Amp and Kan resistence genes ++) to be inserted
into the R. meliloti genome 
Helperstrain: DH5alpha pRK2013
And, what frequency of spontanious resistense to Kan and Amp should I
expect?

Gerd



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