Transformation...

George Tsimiklis gtsimi at po-box.mcgill.ca
Mon Sep 7 23:35:57 EST 1998


.... that part makes common sense.  However, I still don't understand how
the MgCl2 and the CaCl2 play a role in this destabilization of the
membrane.  Is the role of adding MgCl2 or CaCl2 understood, or is it done
simply because it works ... without a known scientific reason?

George Tsimiklis

Elena Sanchez-Heras wrote:

> Basiccally the mechanism is as follows: when we make competent cells,
> what we do is to desestabilize the membrane biochemically so we make it
> more liable to penetration. When we transform those competent cells, the
> heat shcok literally make holes in that pretreated membrane, so the
> foreign DNA can penetrate in the cell.
>
> Elena Sanchez-Heras
>
> On Sat, 5 Sep 1998, George Tsimiklis wrote:
>
> > I would like to know the mechanism of transformation of competent
> > bacteria.  What is the purpose of the heat shock?  Biochemically, how
> > does MgCl2 and CaCl2 increase the efficiency of the transformation?
> > Do they have an effect on bacterial transport proteins?
> >
> >
> >
> >






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