TAvector dephosphorylated?

Markus Schneemann schneema at cmgm.Stanford.EDU
Thu Sep 10 13:43:29 EST 1998


Hi all:

I'm about to generate my own TA-vector for PCR product cloning.
I'll digest pXcmkn12 with XcmI. But then I do not know whether I have to
dephosporylate in order to prevent religation.
Has anybody of you done that before ?
Did you use Boeringer's shrimp alkaline phosphatase (easier to deactivate
later) or any ordinary CIP ?

Cheers,

Markus




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