bacteriophage isolation

Katarina Eskils NOSPAMKatarina.Eskils at mibi.su.se
Fri Sep 11 08:31:50 EST 1998


In article <35F8A041.3CD3973D at uhura.cc.rochester.edu>, Joshua Diamond
<jd007f at uhura.cc.rochester.edu> wrote:

> I have to isolate and characterize a bacteriophage specific for a gram
> positive organism.  Does anyone have any suggestions on where to begin?
> 
> thanks

Try with the bacterium's natural habitat, soil or milk or whatever your
Gram+ originates from. Or, if you already have problem with the phage and
it's temperate, try to force it into the lytic cycle.  Then propagate it
on the bug.
The method depends what the phage source is.

Good luck!
Katarina
-- 
Stockholm, Sweden
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