DNA isolation/ Staph aureus

Crystal D. Ray (BIO) cray1 at chuma.cas.usf.edu
Wed Apr 28 09:32:31 EST 1999


Does anyone have a good protocol for isolating genomic DNA from S. aureus?
I have tried alkaline lysis, boiling lysis, lysozyme.  I would like to try
lysostaphin to lyse my cells, but I need a good protocol.  Any
suggestions?




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