MIC Help

[john Bongiovanni]

Trying to develope new microdilution method for MIC.  Section 6.2.2 if
the ANSI/NCCLS Vol. 17 states "If the inoculum is to added by pipet as
described in section 6.3.2.2, the antimicrobial solutions are prepared
the at twice the desired final concentration and the wells are filled
with 0.05 mL instead of 0.1 mL."

Q - Are both the antimicrobial substrate and broth  made double strength
or is just the antimicrobial?
Thanx in advance
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