Conjugation

MOUNIR IZALLALEN aah313 at agora.ulaval.ca
Tue Mar 2 16:54:02 EST 1999


HI

I'm trying to mobilize back a genomic bank from E.coli to Rhizobium. My
plasmid cannot replicate in Rhizobium and hence I'm selecting for
recombinant containing also the plasmid (Ampicillin resistance). 

My question, beside the ratio, how much recipient and donor should I mix? 

Thanks,

Mounir





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