Need advice on resolving bacteriophage mix

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Mar 8 12:32:53 EST 1999


In article <36E0451B.A7435F48 at sph.unc.edu>, John Scott Meschke
<jmeschke at sph.unc.edu> writes
>I am currently having a problem resolving a mixture of male-specific RNA
>coliphages from male-specific DNA coliphages.  I am interested in
>purifying the RNA phage.  I have tried to plaque purify (3 times in
>sequence), as well as plaque purifying using a composite antisera
>against prototype F+ DNA coliphages (again three times in sequence) with
>no luck. It seems to me that these techniques should have worked ( I
>would have bet that I would have better odds of winning the lottery than
>failing with both techniques).  Please give me any insight you may have
>regarding the persistence of this mix or anyway to resolve it.  Thank
>you very much in advance.
>
>Scott Meschke
>
>

Maybe passage through a host containing a type II restriction enzyme
system would eliminate the DNA phages. That is assuming that they have
one or more sites for the RE. 

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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