Test for catalase

Fergus McClelland redux at perdrix.demon.co.uk
Sat Mar 13 19:10:22 EST 1999


"C Jamison" <jamisoncsnospam at atallaccessam.com> wrote:

>
>yvesch wrote in message <01be67e4$f2e5bf60$4dae9a8e at xbwesrtn>...
>>
>>
>>Hi,
>>
>>    When I make a test for Catalase what percentage of Hydrogen Peroxide
>>use???
>>1%, 2%.... I don't know.
>>
>>Tanks a lot
>>
>>Yves Champagne  tech lab microbiology
>>
>>Montréal, Québec Canada
>
>
>Start with commercial 30%, measure the actual amount spectrophotometrically,
>and dilute to working dilutions (use the suggestions of the group, depending
>on the organism).  Hydrogen peroxide is unstable and tends to degrade rather
>rapidly, so keep it in the fridge and use care when handling as noted by
>Mick.  The spectrophotometric measurement is critical if you are doing
>quantitative tests.  If you are just flooding a plate with hydrogen peroxide
>and looking for bubbles, use 3-10% so you get a good fast reaction.
>
>Scott
>jamisoncsnospam at atallaccessam.com
>
If I want to test a sample of expressed prostatic secretion which is
heavily laden with what looks like a Staph, (Gram + diplococci) and
want to know if it is catalase +ive or -ive, what would be the
quickest and simplest way please?





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