Legionella

lamb L.A.M.Buisman at cable.A2000.nl
Sun Mar 14 11:35:19 EST 1999


Latest news: 34 confirmed cases, 3 dead.
In the Netherlands on average 40 reported cases per year, the majority after a
stay abroad.

Judy/Bob Dilworth wrote:

> The hospital lab I worked in (before being unceremoniously shut down in
> 12/96), sent out urine antigen detections for Legionella to a local
> hospital for testing, but we got results back within 48 hours.  Our
> Ph.D. did DFA staining  for Legionella in our lab.  We made him an air
> dried slide and he took it from there.  I have done DFA's for
> gonorrhoeae (once there is growth on the plate) and they are pretty
> straight forward.  I myself never have read out DFA slides from clinical
> material, but I would think with a monoclonal antibody, if they're
> there, they glow and it's positive.  You would obviously need a
> fluorescent microscope.  Just because there are 96 available wells
> doesn't mean you have to use every well.  We use EIA for C. difficile
> and break off as many wells as we need that day (anywhere from 3 to
> whatever).  Ten years ago, I believe I heard the figure bandied about
> that if you don't do at least ten tests as week (or was it a month????)
> it wasn't worth doing in- house.  Obviously in these days of reduced
> reimbursements you'd have to figure your costs pretty closely as that
> figure could be higher or lower depending upon the test (and what deal a
> lab rep will give you on volume).
>
> Judy Dilworth, M.T. (ASCP)
> Microbiology
>
> lamb wrote:
> >
> > Does anyone know methods for fast lab-diagnosis? IFA on sputumsmears not
> > very reliable.
> > Anybody experience with antigen-testing in urine. I read about EIA, but
> > in 96-well format, and that's a bit much.
> > Loes
> >
> > lamb wrote:
> >
> > > In Holland there has been an outbreak of L. pneumophila infections.
> > > Two patients died, at least another 20 in hospital.
> > > Cause probably a waterfountain in a flower-exhibition.






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