B.cereus

ashwin_k18 at my-dejanews.com ashwin_k18 at my-dejanews.com
Mon Mar 29 21:30:06 EST 1999


In article <7dm4ao$jm8$1 at nnrp1.dejanews.com>,
  aboo9422 at my-dejanews.com wrote:
> I have a question about B.cereus.
>
> I have to count and identify colonies of B.cereus in egg-and-brandy liqueur
> (eggs, sugar and 14 - 20 % alcohol). The medium I use is according to Mossel.
> Now I have the following questions:
>
> 1. are the colonies of B.cereus only vegetative forms or also comming from
> spores.
   B.cereus does form spores and so colonies might contain spores but this
would depend upon the time of incubation and the composition. If uncubation
 is for a short while and medium is at optimal condition then i doubt if there
 would be any sporulation
> 2. if so, how do I recognize vegetative forms and colonies comming from
spores
you can test this if you have entire colonies with you. Take a colony on an
 agar slant and keep and give it horrible conditions to live in for a small
amount of time if all the cells die there were no spores or better still
 take a small part of suspected colony and subculture it the next monochrome
 stain it spores do shoe up in monochrome staining as colorless bodies inside
the cell. or use
 a part of the original culture itself and test.

> 3. is it possible to find any vegetative forms of B.cereus in egg-and-brandy
> liqueur after the eggs had a warm treatment of 30 minutes at 50-60 degree
> Celsius.
> Thanks for your help.
>
> Clara
>
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