agarless Nutrient media

Lesley Robertson l.a.robertson at
Wed Mar 31 05:02:46 EST 1999

Nicholas Landau wrote in message <7drbbf$eka at>...
>ashwin_k18 at writes:
>> is it possible to prepare Nutrient media with inorganic gelling agents
and if
>>so please give me some examples. Agar is very expensive and not all labs
>>can afford large quantities of it. Also agar is likely to influence growth
>>some microorganisms.
>The good news is that other gelling agents are available.  The bad news
>is that agar is about the cheapest one.  In addition, organisms exist
>which can degrade all gelling buffers (although no one organisms of
>which I am aware can degrade them all).  Starch, gellatin and "gel-rite"
>can be used to make media.  If you can't afford agar, then it is time
>to start filling out those grant applications!

Of course, you don't have to buy the ultra-pure grades. For some things, we
buy agar in strips and boil it up ourselves (we get much better Bacillus
sporulation on this, for example).
Lesley Robertson

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