LANA Reagent

Yersinia yersinia at CYBERNEX.NET
Fri May 21 19:45:09 EST 1999

Greetings Microbiologists,

2-3 years ago, I worked in a lab where we used a substance called LANA 
Reagent for the purpose of confirming Gram stains. I never saw it before 
or since.

I want to get hold of the stuff now, but I don't know the proper name for 
it (LANA stands for the chemical it consists of).  I recall that I had to 
mix it up in a Tris buffer and to use it, I'd make up three clear little 
tubes (like Vitek tubes), put 0.5 mL of the reagent in each, and run 
three organisms: a Gram positive as neg control, a Gram negative as 
positive control, and the unknown. Within 30 minutes, a Gram negative is 
confirmed by the clear liquid turning yellow; if there is no color change 
in that time, the organism is Gram positive. 

Do any of you know this reagent, what its actual chemical name is, the 
specific Tris buffer formulation and perhaps a company's catalog which 
sells it?

Thank you so much.


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