C:N Ratio and ammonia production

ecoli at cix.compulink.co.uk ecoli at cix.compulink.co.uk
Tue Nov 2 03:59:05 EST 1999

Ammonia production.
It certainly matters what the conditions and organism are but tryptic 
soy broth has little or no CHO.Because of this the organisms need to use 
the proteins for energy and have  a lot of ammonia left over.
The contemporary wisdom reckons that a C:N ratio below 35:1 should 
result in nitrogen release.I have found that it may need to be alittle 
lower than this say 25:1 for reliable N release but tryptic soy is 
probably below this.
Best wishes,
Peter Harris

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