Pete and Anth
pandasol at mobilixnet.dk
Tue Nov 2 15:42:22 EST 1999
Also realise that there are other forms of EDTA-Na2 (eg. EGTA) that may have
better affinities for Mn (although I am not sure myself). The easiest way is to
probably contact someone like the SIGMA technical staff and they would certinaly
tell you which of there products would bind Mn. Alternatively if you are not
overly worried about the other cations in your solutions it may be worth
lookinjg at some kind of cation exchange resin/column.
> Is manganese a divalent cation as is calcuim and magnesium? if so, try a
> little EDTA (0.5mM to 5 mM) and see if that doesnt work. If I am confused,
> and it is not a divalent cation, the please ignore this post !
> PS. when making up EDTA as a stock, you will have to pH it to get it to go
> into solution. EDTA is made up as a 0.5 M solution, but it will not go into
> solution without pHing to about 7.5 or 8 or so. It will not (to my
> knowledge) go into solution at higher concentrations.
> GIBSON wrote in message <7vc698$h8f$1 at ucsnew1.ncl.ac.uk>...
> >I am currently doing my final year research project, for my BSc. I am
> >looking into the effect of manganese on sporulation in Bacillus subtilis.
> >Does anyone know any compound that would specifically interact with free Mn
> >and remove it from solution??
> >Kevin Gibson
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