Help-Efficiency of Plating-Virus

Cecil Hunter chunter at collabo.com
Wed Nov 3 11:37:03 EST 1999


Im not a microbiologist/virologist so pardon me for my ignorance...I'm
using Enterococcus faecium as a host for producing  bacteriaphage.   I
understand there is a method for determining the efficiency for various
strains to produce phage.  From what I understand efficiency of plating is
different than plaque forming units and is measuring the % of virions that
causes plaque for a particular stain.  Is there such a method out there?
How can i get a hold of one?

Thanks

lamb wrote:

> Cecil Hunter wrote:
>
> > I'm interested in finding a good protocol for efficiency of plating.
>
> What do you mean?
>
> Loes
>
> > I'm particulary interested in using the bacteria Enterococcus in my
> > assay.
> >
> > Any help would be appreciated.


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