Help-Efficiency of Plating-Virus

Larry Farrell farrlarr at isu.edu
Wed Nov 3 12:31:55 EST 1999


Relative efficiency of plating compares the number of plaque forming
units found when plating on a particular bacterial strain to that found
when plating that same phage on a standard host strain.  Absolute
efficiency of plating compares the number of pfu to the number of actual
particles/virions present.  The latter requires quantitative electron
microscopy or some other method that can specifically identify and
enumerate virus particles.

Cecil Hunter wrote:

>  Im not a microbiologist/virologist so pardon me for my
> ignorance...I'm using Enterococcus faecium as a host for producing
> bacteriaphage.   I understand there is a method for determining the
> efficiency for various strains to produce phage.  From what I
> understand efficiency of plating is different than plaque forming
> units and is measuring the % of virions that causes plaque for a
> particular stain.  Is there such a method out there?  How can i get a
> hold of one?
>
> Thanks
>
> lamb wrote:
>
>> Cecil Hunter wrote:
>>
>> > I'm interested in finding a good protocol for efficiency of
>> plating.
>>
>> What do you mean?
>>
>> Loes
>>
>> > I'm particulary interested in using the bacteria Enterococcus in
>> my
>> > assay.
>> >
>> > Any help would be appreciated.
>
--
Larry D. Farrell, Ph.D.
Professor of Microbiology
Idaho State University






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