Help-Efficiency of Plating-Virus

Larry Farrell farrlarr at
Wed Nov 3 14:43:02 EST 1999

Relative efficiency of plating compares the number of plaque forming
units found when plating a given phage on a particular bacterial strain
to that found when plating that same phage on a standard host strain.
Absolute efficiency of plating compares the number of pfu to the number
of actual virions/particles present.  The latter requires quantitative
electron microscopy or some other method that can specifically identify
and quantify virus particles.

Cecil Hunter wrote:

>  Im not a microbiologist/virologist so pardon me for my
> ignorance...I'm using Enterococcus faecium as a host for producing
> bacteriaphage.   I understand there is a method for determining the
> efficiency for various strains to produce phage.  From what I
> understand efficiency of plating is different than plaque forming
> units and is measuring the % of virions that causes plaque for a
> particular stain.  Is there such a method out there?  How can i get a
> hold of one?
> Thanks
> lamb wrote:
>> Cecil Hunter wrote:
>> > I'm interested in finding a good protocol for efficiency of
>> plating.
>> What do you mean?
>> Loes
>> > I'm particulary interested in using the bacteria Enterococcus in
>> my
>> > assay.
>> >
>> > Any help would be appreciated.
Larry D. Farrell, Ph.D.
Professor of Microbiology
Idaho State University

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