Fwd: another ingrediants question
yersinia at CYBERNEX.NET
Fri Nov 5 15:14:03 EST 1999
DC McDonald writes,
<what are the ingrediants of Moeller ornthine decarboxylase medium, and
are the puroses of each. I can't this informaion on the web. i can only
find online shops that sell it. Are there any good links that help
explain these procedures for identification of unknown organisms??
Links, I don't know about, but if you want the information I can help you
a little. According to my Difco Manual (on page 269), the base
decarboxylase medium consists of:
Bacto Peptone, 5 g
Bacto Beef Extract, 5 g
Bacto Dextrose, 0.5 g
Bacto Bromcresol Purple, 0.01 g
Cresol Red, 0.005 g
Pyridoxal, 0.005 g
a 1% solution of L-lysine, L-ornithine or L-arginine.
Ornithine, arginine and lysine decarboxylase reactions are used to
differentiate biochemically "close" spp. of Enterobacteraceae. I have
information on the history and principles of the use of these media and
how they are used to help ID organisms here in my Difco Manual (10th
edition, but I think they've come out with an 11th by now). I'm not
inclined to type all of it out and clog the list with it, though.
However, if you're really interested, I offer the following suggestions:
A. Get your hands on a Difco manual (borrow from work or school lab, or
library if possible, or better, purchase your own copy).
B. Email me privately and I'll send it to you in a private email.
More information about the Microbio