primer melting point?

[Per Mygind]

daniboyton at my-deja.com wrote:

> Can anyone please tell me what the melting points for GC and AT pairs in
> primers are. I believe they are 2 and 4 degrees respectively but I could
> be wrong.                            Thanks
>
> Sent via Deja.com http://www.deja.com/
> Before you buy.

Rule of thomb: 1 degree for every hydrogenbond, that means 2 and 3 degrees,
respectively

--

Yours sincerely

Per Mygind

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Per Mygind, Cand.scient

Department of Medical Microbiology and Immunology
The Bartholin Building, University of Aarhus, Denmark
phone : 89 42 17 47, fax   : 86 19 61 28

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