primer melting point?

[Ulrich Maier]

On Wed, 24 Nov 1999 08:48:26 +0100, Per Mygind <perm at biobase.dk>
wrote:

>daniboyton at my-deja.com wrote:
>
>> Can anyone please tell me what the melting points for GC and AT pairs in
>> primers are. I believe they are 2 and 4 degrees respectively but I could
>> be wrong.                            Thanks
>>
>> Sent via Deja.com http://www.deja.com/
>> Before you buy.
>
IMHO 2 degrees for AT and 4 degrees for GC,
try 4 degrees below the calculated value for first experiments.

should be correct.

Yours, 
Uli Maier 
(7 years PCR practise)


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