primer melting point?

Ulrich Maier maier_hml at
Wed Nov 24 10:09:24 EST 1999

On Wed, 24 Nov 1999 08:48:26 +0100, Per Mygind <perm at>

>daniboyton at wrote:
>> Can anyone please tell me what the melting points for GC and AT pairs in
>> primers are. I believe they are 2 and 4 degrees respectively but I could
>> be wrong.                            Thanks
>> Sent via
>> Before you buy.
IMHO 2 degrees for AT and 4 degrees for GC,
try 4 degrees below the calculated value for first experiments.

should be correct.

Uli Maier 
(7 years PCR practise)


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