plasmid construction

Yong Jiang yjiang at BIOMAIL.UCSD.EDU
Mon Nov 29 13:35:32 EST 1999


Hi,  I just construct a plasmid, and the vector fragment and DNA fragment
was cut by EcoRI, and ran to the agarose gel,  recovered by Gene Clean kit.
The vector was also phosphorylated. They were ligated by Gibco ligase using
a standard ligation protocol, and transformed DH5, and got nothing, even
the positive and negative control worked well. Does anyone have good idea
about it. Thanks a lot.
yong
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Yong Jiang, Ph.D
Department of Biology, University of California (San Diego)
La Jolla, CA 92093-0322
TEL: (858)874-2423 (H); (858)534-2460 (L)
Fax: (858)534-0559 (L)
Email: yjiang at biomail.ucsd.edu
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