New system for isolation of E Coli 0157 from IDG
damian.bond at virgin.net
Fri Oct 8 13:24:16 EST 1999
It depends upon local distributor prices, but should be around £1.00 (UK)
end user price per sample.
"michelle kerr" <kerrmichel at HOTMAIL.COM> wrote in message
news:19991007173535.77628.qmail at hotmail.com...
> How expensive will the Captivat0 be?
> >From: "Liz Middleton" <liz.middleton at lewis-associates.co.uk>
> >To: microbio at net.bio.net
> >Subject: New system for isolation of E Coli 0157 from IDG
> >Date: Thu, 07 Oct 1999 11:02:47 +0100
> >IDG has introduced Captivate O157, an alternative system for the
> >of E.coli O157. The system comprises specific antibody-coated magnetic
> >for the isolation of E.coli O157 from food, animal feed, beverages,
> >pharmaceutical or environmental samples.
> >E.coli O157:H7 is a potentially life-threatening gastrointestinal
> >The majority of infections are foodborne and associated with ground beef,
> >hamburgers and raw milk. Conventional isolation methods can give rise to
> >false negative results due to the problem of competing flora obscuring
> >E.coli O157 colonies.
> >The recommended protocol employs enrichment in Modified Tryptone Soy
> >(MTSB) followed by separation with Captivate, prior to sub-culture of
> >O157-bead complex on Sorbitol MacConkey Agar supplemented with cefixime
> >potassium tellurite (CT-SMAC). E.coli O157 colonies are identified and
> >confirmed with biochemical and serological tests.
> >Immunomagnetic separation offers a way of rapidly extracting E.coli O157
> >from non-target bacteria, resulting in improved rates of isolation. Other
> >benefits include a 10-fold concentration of E.coli O157 and easy to
> >visualise separation of beads. The Captivate separation is highly
> >strain-specific and results in less than 1% carry over of non-O157 E.coli
> >from mixed cultures.
> >Captivate is available in packs of both 50 and 250 tests.
> >- ends
> >Editor: For more information, please contact: Phil Smith, IDG (UK) Ltd,
> >Topley House, 52 Wash Lane, Bury, Lancs, BL9 6AU
> >Phone: +44 (0) 161 797 5729, Fax: +44 (0) 161 762 9322
> >Email: pasmith at idgplc.com
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